Kothe Group

Email: ute.kothe@uleth.ca

Webpage: http://scholar.ulethbridge.ca/kothe

Biochemistry of ribosome biogenesis and tRNA maturation

Ribosome biogenesis is a complex assembly process generating the cell’s protein factories consisting of four large RNAs and more than 50 proteins. As ribosomes are of critical importance for each cell, alterations in ribosome biogenesis lead to human diseases. In order to better understand ribosome formation on a molecular level, our research focuses on the early steps of this process where only a small number of factors are involved. In particular, we study the modification of functional RNAs such as ribosomal and transfer RNA both in bacteria and eukaryotes.
By using highly purified components, we re-create this process under defined conditions in the test tube with the aim of identifying critical, limiting steps. Our approach is multi-disciplinary including molecular biology, biochemistry, biophysics and genetics allowing us to gain detailed insight into the functional cooperation of proteins and RNA in the assembly of cellular machines. Within the Alberta RNA Research and Training Institute (ARRTI), we belong to the Laboratory for structure and function of small noncoding RNAs as well as the Laboratory for biomolecular design and engineering.
 

Group members (Spring 2018)

  1. Dr. Anne Rintala-Dempsey (Research Associate)
  2. Dr. Govardhan Reddy Veerareddygari (PDF)
  3. Dr. Julia Guegueniat (PDF)
  4. Erin Kelly (MSc Student)
  5. Sarah Schultz (MSc Student)
  6. Dominic Czekay (MSc Student)
  7. Elijah Dueck (MSc student)
  8. Hope Vienneau (Undergraduate)
  9. Keara Cheredaryk (Undergraduate)
  10. Jeff McDonald (Undergraduate)
  11. Emily Soon (Research Technician)
  12. Saskia Funk (Visiting Student)

Former Lab Members

  • Keara Cheredaryk - Undergraduate: Summer 2017
  • Anileen Pageni - HYRS: Summer 2015; Undergraduate: Summer 2017
  • Tim Vos - Undergraduate: Summer 2014, Summer 2016, Spring - Summer 2017
  • Abeer Ogailan – MSc Student: Spring 2015 - Summer 2017
  • Amy Hendrickson - Undergraduate: Spring 2017
  • Dr. Anne-Sophie Tillault - PDF: Spring 2014 - Fall 2016
  • Laura Keffer-Wilkes – PhD Student: Spring 2010 - Summer 2016
  • Erin Kelly - HYRS: Summer 2010; Undergraduate: Summer 2012, Summer 2013 (NSERC USRA), Spring 2014, Fall 2014, Spring - Fall 2016 (Honour’s thesis, NSERC USRA)
  • Sarah Schultz - (Undergraduate: Fall 2014 - Summer 2016 (Honour’s Thesis, Chinook Summer Research Award, NSERC USRA)
  • Dominic Czekay - Undergraduate: Spring - Fall 2014 (Chinook Summer Research Award), Fall 2015 - Summer 2016 (Honour’s Thesis)
  • Elijah Dueck - Undergraduate: Summer 2014 (NSERC USRA), Summer 2015 (NSERC USRA), Summer - Fall 2016 (Honour’s Thesis, Chinook Summer Research Award), Summer 2017
  • Jessica Baedke - Undergraduate: Summer 2015 - Summer 2016 (Honour’s Thesis, NSERC URSA)
  • Alycia Amatto - Undergraduate: Summer 2014 (AIHS Summer Studentship), Summer 2015 (AIHS Summer Studentship), Summer 2016 (NSERC USRA)
  • Lindey Felske - Undergraduate: Summer - Fall 2016 (Chinook Summer Research Award)
  • Jordan Stewart - Undergraduate: Spring 2015
  • Kody Beler - Undergraduate: Spring 2015
  • Jan Tuescher - Undergraduate: Fall 2014
  • Melanie Schwerdtfeger - Undergraduate: German Exchange Summer/Fall 2014
  • Sally Xu - HYRS: Summer 2014
  • Chris Cote - Undergraduate: Fall 2013 - Spring 2014
  • Suneet Kharey - Undergraduate: Fall 2013
  • Saskia Funk - RISE German Exchange: Summer - Fall 2013)
  • Jenna Friedt - MSc Student: Fall 2011 - Summer 2013
  • Rajashekhar Kamalampeta - PhD Student: Fall 2008 - Spring 2012

Some Recent Successes:

  • 2015 RiboWest Poster Awards to Jessica Baedke (Undergraduate) and Sarah Schultz (Undergraduate)
  • Ute Kothe awarded Tier II Strategic Chair in Transcriptomics of RNA modification by AITF (2015-2019)
  • CIHR Synapse Mentorship Award 2012 - Individual Researcher, $ 5,000, for Ute Kothe in recognition of science outreach activities and organizing RiboWest Conferences
  • Poster award at RiboWest conference for Jenna Friedt
  • 1st Presentation Award for graduate students in Biochemistry for Jenna Friedt at 6th annual Chinook Symposium for Chemistry & Biochemistry 2012
  • Loren Hepler Gold Medal for PhD Research Excellence, second place, for Laura Keffer-Wilkes at 6th annual Chinook Symposium for Chemistry & Biochemistry 2012